Philippine Science Letters
vol. 3 | no. 1 | 2010
published online May 28, 2010


ARTICLE


A partial expressed sequence tag (EST) library of the economically important red alga Eucheuma denticulatum (N. L. Burham) F. C. Collins and Hervey


by Paulina S. Aspilla1,2, Anna Angela Camille B. Antonio1, Giuseppe C. Zuccarello3, Nina Rosario L. Rojas1,*

1 Department of Chemistry, School of Science and Engineering, Ateneo de Manila University, Loyola Heights, Quezon City, Philippines 1108
2 Department of Chemistry, Silliman University, Dumaguete City, Philippines
3 School of Biological Sciences, Victoria University of Wellington, PO Box 600, Wellington, 6140 New Zealand



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A library of expressed sequence tags (ESTs) was derived from the economically important Rhodophyta species Eucheuma denticulatum . This small scale EST library represents the first look at the set of genes expressed in any Eucheuma species. A total of 311 clones were analyzed. These 311 sequences clustered into 143 unigenes. Thirty-two of the 143 unigenes showed sufficient similarity to known genes to allow annotation. Of these 32 unigenes, 22 were found in at least one other EST collection from Rhodophyta species. Sixteen of the unigenes showed strong similarity to known genes, with e-values smaller than 1x10-14. Another 16 unigenes could be annotated by relaxing the e-value cut-off to 5x10-4. The identified genes covered general metabolic processes (10); translation (2); protein folding, transport, and degradation, including heat shock proteins (6); DNA and RNA binding (2); transcription factors (2); signal transduction, and protein binding (2); cell cycle and apoptosis (2); ion transport (1); and cell membrane, cell wall or extracellular matrix-associated proteins, and other structural proteins (5). Most of the unigenes (111 of them, representing 77.6% of the unigenes), showed no significant similarity to known genes, although 15 of the 111 showed similarity to hypothetical or predicted proteins or raw genomic sequences. Majority of the unannotated unigenes (93 or 64.6% of the library) have not been found in the other Rhodophyta EST libraries, suggesting that many novel genes may still be uncovered by small scale EST collections.

*Corresponding author
Email Address: nrojas@ateneo.edu
Submitted: February 28, 2010
Revised: May 11, 2010
Accepted: May 11, 2010
Published: May 28, 2010
Editor-in-charge: Eduardo A. Padlan