The effect of different media replenishment or in vitro irrigation techniques on seedling growth of ‘Laguna Tall’ coconut seedlings in vitro was studied. Likewise, the effect of in vitro acclimatization techniques using polyethylene glycol and polyvinylpyrolidone on growth and survival of coconut seedlings after acclimatization was investigated. In addition, the effect of three ex vitro acclimatization techniques and potting-out at two different stages of seedling growth on the survival and growth of coconut seedlings was studied. Replenishment of the medium or in vitro irrigation every 60 days and regular subculture every 30-40 days significantly promoted plant height and development of secondary roots. The two in vitro irrigation techniques significantly decreased the percent contamination of cultures. Polyethylene glycol (PEG, 10 and 20 mg L-1) and polyvinylpyrolidone (PVP, 10 and 20 mg L-1) significantly reduced the number of secondary roots and expanded leaves in vitro and the plant height and leaf production ex vitro. Acclimatization using the plastic tent and the wooden box humidity chamber gave higher seedling survival than the misting method. Furthermore, potting-out at two different stages of seedling development (i.e. two- and three-leaf stage) did not significantly affect growth and survival of seedlings after acclimatization.