Philippine Science Letters
vol. 4 | no. 2 | 2011
published online September 15, 2011


Probiotics from Philippine dairy products are bactericidal for pathogens with transferable multiple drug resistance

by Mary Catherine A. Mercado and Esperanza C. Cabrera*

Biology Department and Center for Natural Science and Ecological Research De La Salle University, 2401 Taft Ave., Manila



Probiotic bacteria isolated from locally available commercial dairy products, namely, Nestle Chamyto Probiotic Drink, Yakult Probiotic Drink, Nestle Cerelac Infant Cereal, and Nido 3+ Powdered Milk were established to have antimicrobial activities using the agar spot assay. Tester strains used were extended-spectrum ▀-lactamase (ESBL)-producing strains of Enterobacter aerogenes, Klebsiella pneumoniae, Proteus mirabilis, and Escherichia coli, metallo-▀-lactamase (MBL)-producing strain of Pseudomonas aeruginosa, and three strains of methicillin resistant Staphylococcus aureus (MRSA). The probiotics inhibited the growth of all the tested multiple drug resistant strains. Further tests showed that the isolate from Chamyto displayed bactericidal activities on all the test strains. Yakult and Nestle Cerelac probiotics were bactericidal for all strains except for ESBL-producing E. coli. Nido 3+ probiotic isolates were bactericidal for all strains except for ESBL -producing K. pneumoniae. The probiotic isolates were identified phenotypically and through 16S rDNA analysis to be Lactobacillus casei or Lactobacillus paracasei. The in vitro bactericidal activities of the isolated probiotics show great potential for further research in treatment and prophylaxis of infections from multiple drug resistant bacteria.

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Submitted: February 27, 2011
Revised: July 6, 2011
Accepted: July 19, 2011
Published: September 15, 2011
Editor-in-charge: Francis de los Reyes III