Philippine Science Letters
vol. 5 | no. 2 | 2012
published online September 28, 2012


Ascaris suum infective eggs upregulate IL-4, 5 and 10 in BALB/c mice

by Maria Ruth B. Pineda1,2,4*and John Donnie A. Ramos1,3,4

1UST The Graduate School,
2Department of Medical Technolgy, Faculty of Pharmacy,
3Department of Biological Sciences, College of Science,
4Research Center for the Natural and Applied Sciences, University of Santo Tomas, Manila,



Experimental animals such as BALB/c mice are invaluable models in the elucidation of immune responses in parasitic infections. The present study determined the effect of the infective eggs of Ascaris suum in BALB/c mice through the measurement of cytokine and immunoglobulin levels. Thirty BALB/c mice were randomly grouped into normal control group (NC) and Ascaris group (AS), divided into three trials (5 mice per group per trial). Those in the AS group were chronically infected with Ascaris suum (As) eggs, while those in the NC group were given placebos. At days 0, 36 and 72, stool samples were collected for fecalysis and formalin ether concentration technique (FECT) while blood samples were extracted and used for ELISA for the measurement of cytokines and As-specific antibodies. Liver, lungs and small intestines were harvested for histopathologic analysis. Chronic Ascaris suum infection in BALB/c mice significantly increased the levels of IL-4, IL-5, and IL-10. It did not significantly change the level of As-specific IgE but slightly increased the level of As-specific IgG. Stool specimens from all mice showed negative fecalysis and FECT results. Pathological conditions were observed from the liver, lungs and small intestines of infected mice. Ascaris suum is capable of infecting BALB/c mice, which indicates that it can cross species barrier. Immune response of mice was modulated through production of IL-4, IL-5 and IL-10 and As-specific IgG.

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Submitted: April 1, 2012
Revised: June 25, 2012
Accepted: July 17, 2012
Published: September 28, 2012
Editor-in-charge: Eduardo A. Padlan