Philippine Science Letters
vol. 5 | no. 2 | 2012
published online December 19, 2012


ARTICLE


Two strains of Gordonia terrae isolated from used engine oil contaminated soil utilize short- to long-chain n-alkanes


by Michael Angelo C. Nicdao1,2 and Windell L. Rivera 1,3,*


1Institute of Biology, College of Science, University of the Philippines, Diliman, Quezon City 1101, Philippines
2Institute of Arts and Sciences, Pampanga Agricultural College, Magalang, Pampanga 2011, Philippines
3Natural Sciences Research Institute, University of the Philippines, Diliman, Quezon City 1101, Philippines




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This study aimed to isolate bacteria that are able to degrade a wide range of hydrocarbon (HC) compounds. Samples were taken from soil contaminated with engine oil using mineral salt medium (MSM). Taxonomic classification of two isolates, using the Biolog Microbial Identification System, revealed that they are actinomycetes belonging to the suborder Corynebacterineae. Nucleotide sequences from amplified 16S ribosomal RNA (rRNA) gene and phylogenetic analysis showed that the isolates (designated as G1 and G2) are two different strains of Gordonia terrae. The isolates are capable of degrading HCs specifically n-alkanes as demonstrated by HC utilization fingerprints and gas chromatography-mass spectrometry (GC-MS) experiments. Their capability to grow on a wide range of saturated HCs is attributed to the presence of the alkane hydroxylase (alkB) gene. The isolation of these bacteria has added the limited number of species that are able to degrade short- to long-chain saturated alkanes.

*Corresponding author
Email Address: wlrivera@science.upd.edu.ph
Submitted: July 21, 2012
Revised: October 30, 2012
Accepted: November 12, 2012
Published: December 19, 2012
Editor-in-charge: Gisela P. Padilla - Concepcion
Reviewers:
Diana S. Aga
Francis L. de los Reyes III
Asuncion K. Raymundo