Philippine Science Letters
vol. 3 | no. 2 | 2010
published online December 14, 2010


DNA barcoding of the White-Collared Kingfisher Todiramphus chloris (Boddaert 1783) (Alcedinidae) using the mitochondrial cytochrome c oxidase subunit I gene

by Adrian U. Luczon*, Abdel Hadi M. Mohammad Isa, Jonas P. Quilang,
Perry S. Ong, Ian Kendrich C. Fontanilla

DNA Barcoding Laboratory, Institute of Biology, University of the Philippines, Diliman 1101, Quezon City, Philippines



The White-Collared Kingfisher (Todiramphus chloris) is a resident Philippine bird species. In accordance with the objective of the All Birds Barcoding Initiative (ABBI) to barcode all bird species in the world, this study reports the first barcodes of T. chloris using the mitochondrial gene cytochrome c oxidase subunit I (COI). COI sequences from this species as well as from other members of the family Alcedinidae available in Genbank and the Barcodes of Life Data (BoLD) Systems were compared in order to test for the utility of COI to delineate species. Monophyly of the species was established, supporting the use of barcodes for species discovery. Sequences between T. chloris and T. sanctus, however, revealed a close association between the two species which warrants further taxonomic review.

*Corresponding author
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Submitted: July 11, 2010
Revised: November 22, 2010
Accepted: November 25, 2010
Published: December 14, 2010
Editor-in-charge: Eduardo A. Padlan